Journal: Advances in Experimental Medicine and Biology

Article Title: Antimicrobial Peptides

doi: 10.1007/978-981-13-3588-4

Figure Lengend Snippet: Fig. 2.1 Correlation between membrane permeabiliza- tion and bacterial death. Magainin 2 (50 nmol) was added to E. coli cells (5 × 108 CFU/mL). K+ efflux (open circles, left axis) and the percent cell viability (closed circles, right axis) are plotted as a function of time

Article Snippet: First, by partially incorporating D-amino acids, we obtained GF-17d3 (Li et al. 2006a), which is only active against Gramnegative E. coli ATCC 25922 and A. baumannii B28-16, but not Gram-positive pathogens we tested (Wang et al. 2017).

Techniques: Membrane

Journal: Advances in Experimental Medicine and Biology

Article Title: Antimicrobial Peptides

doi: 10.1007/978-981-13-3588-4

Figure Lengend Snippet: Fig. 13.5 Example radial diffusion-based screening of members of a peptide library in parallel against two organisms using radial diffusion. The same set of peptide library members were screened against the Gram-negative E. coli and the Gram-positive S. aureus with radial diffu- sion. Zones of inhibition are observed for some library members. Some active library members inhibit both bac- teria (yellow), while others inhibit only one of the two

Article Snippet: First, by partially incorporating D-amino acids, we obtained GF-17d3 (Li et al. 2006a), which is only active against Gramnegative E. coli ATCC 25922 and A. baumannii B28-16, but not Gram-positive pathogens we tested (Wang et al. 2017).

Techniques: Diffusion-based Assay, Inhibition

Journal: Communications biology

Article Title: Exploring a general multi-pronged activation strategy for natural product discovery in Actinomycetes.

doi: 10.1038/s42003-023-05648-7

Figure Lengend Snippet: Fig. 1 Schematics of a multi-pronged general activation strategy for native microbial strains toward natural product discovery. A Microbial strains are genetically edited via one-step phiC31 integrase protocol for heterologous expression of genes for activation and/or upregulation of secondary metabolites. B Genetic-based activation is complemented with cultivation-based (one strain many compounds, OSMAC) activation where a range of fermentation media (with varied nutrient compositions) is employed to probe the full biosynthetic potential of the genetically edited mutants. C Extracts are profiled both chemically and with bioassays to characterize and discover new bioactive metabolite space. Bioactivity assays include antibacterial assays (Acinetobacter baumannii, Klebsiella aerogenes, Pseudomonas aeruginosa and Staphylococcus aureus Rosenbach), antifungal assays (Aspergillus fumigatus) and mammalian cytotoxicity assays (A549 human lung carcinoma cells). D In depth analysis of LCMS data allowed us to interrogate the effects of media and activators where we found the combination of 3 regulators and 3 media were sufficient to produce maximal metabolites production. E Bioactivity profile comparison between mutants and native strains revealed that the expanded chemical space was accompanied by increased or novel bioactivities, which were used for new NP discovery.

Article Snippet: 1 and 2 were active against Gramnegative Acinetobacter baumannii (ATCC® 19606TM) (Table S10, Fig. S32), with MIC50 of 9.8 μM and 6.9 μM respectively.

Techniques: Activation Assay, Expressing, Comparison

Journal: Materials science & engineering. C, Materials for biological applications

Article Title: Acid-activated ROS generator with folic acid targeting for bacterial biofilm elimination.

doi: 10.1016/j.msec.2021.112225

Figure Lengend Snippet: Fig. 3. (a) Time-dynamic bactericidal assay images of viable S. aureus and E. coli grown with ZIF-8@Lys-CD@FA at different times in pH 7.4 and pH 5.5 PBS after 24 h of culture. (b) SEM morphology of S. aureus and E. coli seeded on ZIF-8@Lys-CD@FA (0.5 mg mL−1) after incubation at 37 ◦C for control (a, d), 1 h (b, e), 4 h (c, f).

Article Snippet: The antibacterial activities against E. coli (ATCC 25922, Gramnegative) and S. aureus (ATCC 25923, Gram-positive) were characterized by a minimum inhibitory concentration (MIC).

Techniques: Serum Bactericidal Assay, Incubation, Control